For my final rotation in the clinical laboratory, I was placed in the chemistry lab. This branch of the lab deals with a large majority of the tests routinely ordered in a hospital, such as glucose, electrolytes, etc. Continue reading
The clinical hematology laboratory contains two different types of testing: hematological and coagulation testing. On the hematological side of testing, the main test performed is the complete blood count (CBC), which directly measures the number of red blood cells, white blood cells, platelets, and hemoglobin, as well as indirectly measuring other values by calculations (such as the mean corpuscular hemoglobin). These tests are performed using a well-mixed whole blood sample that has been collected in a tube containing ethylene diamine triacetic acid, also known as EDTA. Before hematological results are sent out to the healthcare team, they are reviewed by the medical laboratory technologist (MLT). Any abnormal results that are seen by the MLT (either increased or decreased beyond reference ranges) are made into slides and further microscopic analyses are performed. Some results may even be “critical” in the sense that they are so abnormal that the patient may be at risk of harm if the result is not reported immediately. While reviewing parameters, certain “checks” are also performed as an internal quality control system for the sample. One of these kinds of systems include delta checks, which are a comparison of a patient’s previous results to those most current. While changes in values can occur due to the physiological state of the patient, these internal checks are also great for identifying if a sample was taken from the wrong patient. In addition, H&H checks are performed between the patient’s hemoglobin and hematocrit. The hematocrit should match the hemoglobin within certain values, and if not, an issue with the specimen quality is present, and this should be addressed before results are reported for that patient.
One of the main reasons why I chose the medical laboratory profession was for the behind-the-scenes work in the medical field. Nevertheless, I am thankful for those individuals on the front line of the medical profession, directly taking care of patients and their illnesses. Even though this may have the initial reason that drew me to the medical laboratory profession, I have found, throughout my years in university, that I have many other qualities belonging to that of a medical laboratory technologist: I am analytical, process-driven, and a critical thinker. Nonetheless, there has been one aspect of my program that had me a bit worried… Continue reading
The Transfusion Medicine laboratory is responsible for the testing and storing of blood products that will eventually be transfused into patients. Many people are familiar with traditional blood transfusions, however, the Transfusion Medicine laboratory carries many other types of blood products that are requested to be transfused into patients, such as platelets, fresh frozen plasma, albumin, etc. Continue reading
For my third rotation in the clinical laboratory, I was in the Transfusion Medicine laboratory. For those who are unfamiliar with this section of the lab, it is responsible for the testing of patients’ blood and blood products before transfusions occur. Continue reading
Throughout my final weeks in the Histology lab, I performed a variety of special stains in order to detect connective tissues, carbohydrates, microorganisms, and pigments in the diagnosis of certain diseases. I also performed immunohistochemistry in the detection of cancer markers. In a routine hematoxylin and eosin stain, many of these components are not visible and therefore special stains are needed in order for them to be seen.
Throughout this week in the Histology lab, I was operating the automated hematoxylin and eosin stainer, while also submitting stained slides to the pathologists to be reviewed.
Hematoxylin and eosin is a stain used in routine Histology slides. The hematoxylin stains nuclei in the tissue blue, whereas eosin stains the cytoplasm and other structures varying shades of pink. Hematoxylin is a natural dye originating from the logwood tree, Haematoxylum campechianum. However, it is not hematoxylin that is truly staining the nuclei, but rather its oxidized state of hematein. Oxidation of hematoxylin to hematein can occur using two different methods. One is a natural oxidation process that uses air or light. This process takes a significant amount of time to oxidize the dye, however, it produces a very stable stain. The other method uses chemical oxidation that requires either sodium iodate or potassium iodate. While this process occurs much quicker than the natural method, these stains may deteriorate and require filtering before use. However, this oxidized state of hematoxylin is still not sufficient to stain tissues. Hematoxylin must also be combined with a ‘mordant’, which gives the dye affinity to the tissue. Typical mordants that are added to hematoxylin are aluminum, iron, lead, tungsten, and molybdenum. Continue reading